Plant Transcription Factor Database
Previous version: v3.0
Thellungiella parvula
HSF Family
Species TF ID Description
Tp1g28570HSF family protein
Tp1g34200HSF family protein
Tp2g08640HSF family protein
Tp2g10600HSF family protein
Tp2g25190HSF family protein
Tp3g02050HSF family protein
Tp3g20530HSF family protein
Tp3g22310HSF family protein
Tp4g06150HSF family protein
Tp4g23930HSF family protein
Tp5g00190HSF family protein
Tp5g10360HSF family protein
Tp5g22990HSF family protein
Tp6g05810HSF family protein
Tp6g12060HSF family protein
Tp6g27310HSF family protein
Tp6g39000HSF family protein
Tp7g11770HSF family protein
Tp7g16360HSF family protein
Tp7g17540HSF family protein
Tp7g17590HSF family protein
Tp7g34770HSF family protein
Tp_un0226_001HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645